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Use of Antibodies In Small Animal Molecular Imaging
18 Aug 2010: In vivo imaging enables the natural course of a disease or experimental model to be monitored. This application note discusses the use of fluorescently labeled primary antibodies for looking at protein expression in vivo imaging. The use of antibodies allows researchers to quickly determine if the test antibody begins to target undesired organs such as the kidneys or livers.
Carestream Molecular Imaging
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Recent articles: |
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Characterization of Ion Channels in Stem Cell-derived Cardiomyocytes on An Automated Parallel Patch Clamp System
22 Jun 2010: In this application note Molecular Devices use the PatchXpress 7000A automated parallel patch clamp system is used to record Na, K and Ca currents from stem cell-derived mouse cardiomyocytes. The collaborative effort demonstrates that the combination of “ready-to-use” cardiomyocytes with an automated patch clamp system offers a powerful assay platform for fast assessment of compound efficacy and for safety profiling of lead compounds in a biologically relevant system.
Molecular Devices
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Expansion and Differentiation of H7 Human Embryonic Stem Cells in Chemically Defined Medium Using Corning® Synthemax™ Surface
21 Jun 2010: In this application note Corning Synthemax Surface is used to support long-term self-renewal and differentiation of H7 hESCs. Cells demonstrated a stable doubling time, high level of expression of hESC phenotypic markers (Oct4, SSEA4 and TRA-1-60) and normal karyotype. Corning Synthemax Surface also supported successful directed differentiation of H7 cells into functional cardiomyocytes, proving suitability of Corning Synthemax Surface for both expansion and differentiation of hESC culture.
Corning Life Sciences
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Human Oncostatin M is Functionally Equivalent to Mouse LIF in Supporting Mouse ES Cell Pluripotency & Germline Competency
21 Jun 2010: In this application note R&D Systems demonstrate human OSM is able to maintain ES cell pluripotency in a similar manner to mouse LIF, including long-term maintenance of pluripotency and the ability to differentiate in vitro, the ability to activate STAT3, and germline transmission competency. The study suggests that OSM may be an additional factor that plays a role in early embryonic development and that it may be used as an alternative to LIF in mouse ES cell culture.
R&D Systems, Inc.
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Corning® Epic® System: Real-time Monitoring of Cell Response to Virus Infection
24 May 2010: This application note discusses the use of the Epic System, an optical sensor-based label-free HTS platform (1), to monitor real-time the HeLa cell response to human rhinovirus (HRV) infection.
Corning Life Sciences
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Analysis of Changes in RTK Phosphorylation Using Proteome Profiler™ 96 Antibody Arrays
26 Apr 2010: Methods to identify the effect of RTK inhibitors or modifying antibodies on RTK phosphorylation are effective tools for drug screening. This application note presents the Proteome Profiler 96 human Phospho-RTK Antibody Array as a unique and powerful multiplex immunoassay that utilizes ELISA techniques to measure changes in the phosphorylation of multiple RTKs simultaneously.
R&D Systems, Inc.
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Comparison of HTRF® Indirect Systems for Mouse and Rat Monoclonal Antibody Detection
05 Mar 2010: This application note shows the uncontested superiority of specific anti-species antibodies versus Ig-binding proteins. In the series, rabbit anti-mouse polyclonal antibody always improved the detection of mouse and rat monoclonal antibodies. This was also true for the immunodetection of rabbit polyclonal antibodies.
Cisbio Bioassays
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Universal Assays for Serine-Threonine andTyrosine Kinases
04 Feb 2010: The HTRF® KinEASE™ assay platform for screening serine/threonine kinases (STKs) is now extended to Tyrosine kinases (TKs). Developed in collaboration with Millipore, the HTRF® KinEASE™ reagents are ready-to-use kits based on Cisbio’s patented HTRF® technology and validated on over 160 Ser/Thr and Tyr kinases.
Cisbio Bioassays
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HTRF® Human SIRT1 Assay: A New Solution for HDAC/SIRT Screening
30 Jan 2010: The HTRF SIRT1 assay is a homogeneous solution for measuring SIRT1 activity. SIRT1, associated with class III of HDACs, has been identified as a regulator of a variety of biological processes, such as glucose homeostasis, mitochondria biogenesis, adipogenesis, apoptosis, senescence and metabolism.
Cisbio Bioassays
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Binding Kinetics Analysis with SPR: Interaction between Bovine Serum Albumin (BSA) and Anti-BSA
22 Jan 2010: In this application note, the use of flow injection SPR for real-time monitoring of the association and dissociation of anti-bovine serum albumin (anti-BSA) antibody with surface-immobilized BSA molecules is demonstrated. In restriction digestion, BSA has been used to stabilize enzymes during DNA digestion.
Biosensing Instrument
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